Question 1

Direct selection involves inoculating cells onto growth media on which

Accepted Answer

A)  the mutant but not the parental cell type will grow.
B)  the mutation will be reversed.
C)  the nutrients necessary for mutation to occur are present.
D)  the mutagen is present.E) C) and D)
F) A) and B)

Question 2

To maximize the number of thymine dimer mutations following UV exposure, should you keep a plate of fungal cells in the dark, in the light, or does it matter at all?

Accepted Answer

A)  The dark-light will activate the photorepair systems that can break thymine dimers induced by UV light.
B)  The light-it's important to keep on producing the thymine dimers by keeping the plate exposed to light as much as possible.
C)  It's best to alternate light and dark every hour to increase the chances that thymine dimers will form in the fungal cells, but still keep the photorepair systems from correcting them as they are formed.
D)  It doesn't matter-fungal cells don't possess the enzymes needed for photorepair of thymine dimers.They're only in prokaryotes.E) A) and D)
F) B) and C)

Question 3

Insertion sequences

Accepted Answer

A)  are the simplest type of transposon.
B)  code for a transposase enzyme.
C)  are characterized by an inverted repeat.
D)  can produce pili.
E)  are the simplest type of transposon, code for a transposase enzyme AND are characterized by an inverted repeat.F) B) and E)
G) A) and E)

Question 4

Plasmids often carry the information for antibiotic resistance.

Accepted Answer

A) True 
 B)False

Question 5

Indirect selection

Accepted Answer

A)  is necessary to isolate auxotrophic mutants.
B)  uses media on which the mutant but not the parental cell type will grow.
C)  uses media that reverses the mutation.
D)  uses media upon which neither the parental cell type or mutant grows.
E)  is necessary to isolate auxotrophic mutants AND uses media upon which neither the parental cell type or mutant grows.F) All of the above
G) A) and E)

Question 6

X-rays

Accepted Answer

A)  have no effect on DNA.
B)  cause thymine trimers.
C)  cause single and double strand breaks in DNA molecules.
D)  make the DNA radioactive.E) None of the above
F) All of the above

Question 7

Double-stranded DNA enters the recipient cell during transformation.

Accepted Answer

A) True 
 B)False  False

Question 8

Replica plating

Accepted Answer

A)  is useful for direct selection.
B)  is useful for identifying auxotrophs.
C)  uses media on which the mutant will not grow and the parental cell type will.
D)  is used to store strains of bacteria.
E)  is useful for identifying auxotrophs AND uses media on which the mutant will not grow and the parental cell type will.F) None of the above
G) A) and D) E

Question 9

Every 24 hours, every genome in every cell of the human body is damaged 10,000 times or more. Given the possible DNA repair mechanisms, which order listed below would be most effective at repairing these as quickly as possible in order to prevent mutations from being carried forward in DNA replication?

Accepted Answer

A)  proofreading by DNA polymerase, glycosylase enzyme activities, excision repair, photoreactivation, SOS repair
B)  SOS repair, photoreactivation, excision repair, glycosylase enzyme activities, proofreading by DNA polymerase
C)  photoreactivation, SOS repair, proofreading by DNA polymerase, glycosylase enzyme activities, excision repair
D)  glycosylase enzyme activities, SOS repair, photoreactivation, proofreading by DNA polymerase, excision repairE) A) and B)
F) B) and D)

Question 10

Competent cells

Accepted Answer

A)  are able to take up naked DNA.
B)  are antibiotic resistant.
C)  occur naturally.
D)  can be created in the laboratory.
E)  are able to take up naked DNA, occur naturally AND can be created in the laboratory.F) A) and B)
G) A) and E)

Question 11

Mutations

Accepted Answer

A)  may occur spontaneously.
B)  occurring at one gene are often coupled to mutation of another gene.
C)  are more likely to be killed by using 2 antibiotics.
D)  are always harmful.
E)  may occur spontaneously AND are more likely to be killed by using 2 antibiotics.F) B) and C)
G) A) and B)

Question 12

Bacteria that have properties of both the donor and recipient cells are the result of

Accepted Answer

A)  UV light.
B)  SOS repair.
C)  frame shift mutations.
D)  genetic recombination.E) A) and D)
F) A) and B)

Question 13

A clever technique that streamlines the identification of auxotrophic mutants is

Accepted Answer

A)  gas chromatography.
B)  replica plating.
C)  direct selection.
D)  reversion.E) A) and B)
F) A) and C)

Question 14

The Ames test is useful as a rapid screening test to identify those compounds that

Accepted Answer

A)  will respond to chemical agents.
B)  have a high probability of being carcinogenic.
C)  respond to the deletion of DNAses.
D)  will protect an organism from cancer.
E)  will respond to chemical agents AND will protect an organism from cancer.F) A) and B)
G) A) and C) B

Question 15

Chemical mutagens that mimic the naturally occurring bases are called

Accepted Answer

A)  nitrogen mustards.
B)  alkylating agents.
C)  base analogs.
D)  nitrous oxide.E) None of the above
F) C) and D)

Question 16

Among the easiest of the mutations to isolate are those which

Accepted Answer

A)  involve haploid chromosomes.
B)  involve antibiotic resistance.
C)  allow populations to be measured.
D)  use an indirect method for measurement.
E)  involve haploid chromosomes AND involve antibiotic resistance.F) A) and B)
G) A) and E)

Question 17

In conjugation the donor cell is recognized by the presence of

Accepted Answer

A)  an F plasmid.
B)  a Y chromosome.
C)  diploid chromosomes.
D)  an SOS response.
E)  an F plasmid AND diploid chromosomes.F) C) and E)
G) C) and D)

Question 18

Segments of DNA capable of moving from one area in the DNA to another are called

Accepted Answer

A)  base analogs.
B)  intercalating agents.
C)  transposons.
D)  palindromic sequences.E) A) and C)
F) None of the above

Question 19

The largest group of chemical mutagens consists of

Accepted Answer

A)  radiation.
B)  base analogs.
C)  nitrous acid.
D)  alkylating agents.E) None of the above
F) A) and D)

Question 20

Nitrous acid most frequently causes mutations by

Accepted Answer

A)  substituting oxygen for hydrogen bonds.
B)  converting keto groups to amino groups.
C)  altering the bonding ratios of nitrogen bases.
D)  converting cytosine to uracil.E) A) and D)
F) All of the above

Exam 8: Bacterial Genetics

Direct selection involves inoculating cells onto growth media on which

Correct Answer
verified

To maximize the number of thymine dimer mutations following UV exposure, should you keep a plate of fungal cells in the dark, in the light, or does it matter at all?

Correct Answer
verified

Insertion sequences

Correct Answer
verified

Plasmids often carry the information for antibiotic resistance.

Correct Answer
verified

Indirect selection

Correct Answer
verified

X-rays

Correct Answer
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Double-stranded DNA enters the recipient cell during transformation.

Correct Answer
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False

Replica plating

Correct Answer
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E

Correct Answer
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Competent cells

Correct Answer
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Mutations

Correct Answer
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Bacteria that have properties of both the donor and recipient cells are the result of

Correct Answer
verified

A clever technique that streamlines the identification of auxotrophic mutants is

Correct Answer
verified

The Ames test is useful as a rapid screening test to identify those compounds that

Correct Answer
verified
B

Chemical mutagens that mimic the naturally occurring bases are called

Correct Answer
verified

Among the easiest of the mutations to isolate are those which

Correct Answer
verified

In conjugation the donor cell is recognized by the presence of

Correct Answer
verified

Segments of DNA capable of moving from one area in the DNA to another are called

Correct Answer
verified

The largest group of chemical mutagens consists of

Correct Answer
verified

Nitrous acid most frequently causes mutations by

Correct Answer
verified

Exam 8: Bacterial Genetics

Direct selection involves inoculating cells onto growth media on which

Correct AnswerverifiedShow Answer

To maximize the number of thymine dimer mutations following UV exposure, should you keep a plate of fungal cells in the dark, in the light, or does it matter at all?

Correct AnswerverifiedShow Answer

Insertion sequences

Correct AnswerverifiedShow Answer

Plasmids often carry the information for antibiotic resistance.

Correct AnswerverifiedShow Answer

Indirect selection

Correct AnswerverifiedShow Answer

X-rays

Correct AnswerverifiedShow Answer

Double-stranded DNA enters the recipient cell during transformation.

Correct AnswerverifiedFalse

Replica plating

Correct AnswerverifiedE

Correct AnswerverifiedShow Answer

Competent cells

Correct AnswerverifiedShow Answer

Mutations

Correct AnswerverifiedShow Answer

Bacteria that have properties of both the donor and recipient cells are the result of

Correct AnswerverifiedShow Answer

A clever technique that streamlines the identification of auxotrophic mutants is

Correct AnswerverifiedShow Answer

The Ames test is useful as a rapid screening test to identify those compounds that

Correct AnswerverifiedB

Chemical mutagens that mimic the naturally occurring bases are called

Correct AnswerverifiedShow Answer

Among the easiest of the mutations to isolate are those which

Correct AnswerverifiedShow Answer

In conjugation the donor cell is recognized by the presence of

Correct AnswerverifiedShow Answer

Segments of DNA capable of moving from one area in the DNA to another are called

Correct AnswerverifiedShow Answer

The largest group of chemical mutagens consists of

Correct AnswerverifiedShow Answer

Nitrous acid most frequently causes mutations by

Correct AnswerverifiedShow Answer

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False

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E

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B

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