Question 1

Which of the following was first produced commercially using recombinant DNA technology?

Accepted Answer

A)   human growth hormone
B)   interleukins
C)   hepatitis B vaccine
D)   human insulinE) C) and D)
F) A) and C)

Question 2

Regardless of the exact approach taken to recombinant DNA technology, one of the keys to successful cloning is choosing the right vector.

Accepted Answer

A) True 
 B)False

Question 3

When a eukaryotic gene is cloned into a bacterium, the advantage of a complementary DNA (cDNA)  gene being used instead of fragments of genomic DNA is that

Accepted Answer

A)   the promoter and terminator are found in the cDNA gene but not in the genomic fragment.
B)   the introns have been removed from the cDNA gene but not from the genomic fragment.
C)   the cDNA is made with the nucleotides found in the prokaryote but not in the eukaryote.
D)   There is no advantage to using a cDNA gene rather than a genomic fragment.E) B) and C)
F) All of the above

Question 4

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Accepted Answer

A) True 
 B)False  True

Question 5

The PCR method was developed by

Accepted Answer

A)   Boyer.
B)   Mullis.
C)   Cohen.
D)   Sanger.E) C) and D)
F) B) and C)

Question 6

The polymerase chain reaction (PCR)  can be used to produce __________ of copies in a few hours.

Accepted Answer

A)   hundreds
B)   thousands
C)   millions
D)   billionsE) A) and B)
F) B) and D)

Question 7

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Accepted Answer

A)   metabolic activation
B)   antibiotic resistance
C)   insertion sequence
D)   promoter/operatorE) B) and C)
F) A) and D)

Question 8

Which of the following is true about restriction endonucleases?

Accepted Answer

A)   They make a blunt cut on the two DNA strands so that there are no single-strand regions.
B)   They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
C)   Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
D)   Depending on the incubation conditions, the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.E) A) and B)
F) All of the above

Question 9

Which of the following is not true of cloning vectors?

Accepted Answer

A)   They usually contain multicloning sites or polylinkers.
B)   They contain at least two replication origins.
C)   They can be replicated within an appropriate host.
D)   All of these are true of cloning vectors.E) A) and B)
F) B) and D)

Question 10

When a eukaryotic gene is expressed in a bacterium, the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Accepted Answer

A) True 
 B)False

Question 11

Restriction endonucleases are produced by

Accepted Answer

A)   fungi.
B)   bacteria.
C)   protozoa.
D)   plants.
E)   all of the choicesF) None of the above
G) C) and D)

Question 12

The enzyme reverse transcriptase was discovered by

Accepted Answer

A)   Arber and Smith.
B)   Jackson, Symons, and Berg.
C)   Boyer and Cohen.
D)   Temin and Baltimore.E) None of the above
F) B) and C) D

Question 13

Complementary DNA (cDNA)  probes are produced using

Accepted Answer

A)   restriction endonucleases.
B)   RNA polymerase.
C)   DNA ligase.
D)   reverse transcriptase.E) All of the above
F) C) and D) D

Question 14

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 15

Transposons are frequently used as cloning vectors.

Accepted Answer

A) True 
 B)False

Question 16

In order to express eukaryotic genes in a bacterium, the __________ must first be removed.

Accepted Answer

A)   introns
B)   exons
C)   enhancers
D)   3' poly A sequenceE) A) and D)
F) B) and C)

Question 17

Genetic engineering has allowed the useful production of human hemoglobin in

Accepted Answer

A)   monkeys.
B)   dogs.
C)   pigs.
D)   cows.E) All of the above
F) C) and D)

Question 18

Restriction endonucleases were discovered by

Accepted Answer

A)   Arber and Smith.
B)   Jackson, Symons, and Berg.
C)   Boyer and Cohen.
D)   Temin and Baltimore.E) B) and D)
F) B) and C)

Question 19

Recombinant DNA technology does not rely on which of the following enzymes?

Accepted Answer

A)   restriction endonucleases
B)   RNA methylase
C)   DNA ligase
D)   reverse transcriptaseE) B) and C)
F) C) and D)

Question 20

A (n)  __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Accepted Answer

A)   shuttle
B)   chimeric
C)   expression
D)   phageE) B) and C)
F) A) and C)

Exam 15: Recombinant Dna Technology

Which of the following was first produced commercially using recombinant DNA technology?

Correct AnswerverifiedShow Answer

Regardless of the exact approach taken to recombinant DNA technology, one of the keys to successful cloning is choosing the right vector.

Correct AnswerverifiedShow Answer

When a eukaryotic gene is cloned into a bacterium, the advantage of a complementary DNA (cDNA) gene being used instead of fragments of genomic DNA is that

Correct AnswerverifiedShow Answer

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Correct AnswerverifiedTrue

The PCR method was developed by

Correct AnswerverifiedShow Answer

The polymerase chain reaction (PCR) can be used to produce __________ of copies in a few hours.

Correct AnswerverifiedShow Answer

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Correct AnswerverifiedShow Answer

Which of the following is true about restriction endonucleases?

Correct AnswerverifiedShow Answer

Which of the following is not true of cloning vectors?

Correct AnswerverifiedShow Answer

When a eukaryotic gene is expressed in a bacterium, the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Correct AnswerverifiedShow Answer

Restriction endonucleases are produced by

Correct AnswerverifiedShow Answer

The enzyme reverse transcriptase was discovered by

Correct AnswerverifiedD

Complementary DNA (cDNA) probes are produced using

Correct AnswerverifiedD

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Correct AnswerverifiedShow Answer

Transposons are frequently used as cloning vectors.

Correct AnswerverifiedShow Answer

In order to express eukaryotic genes in a bacterium, the __________ must first be removed.

Correct AnswerverifiedShow Answer

Genetic engineering has allowed the useful production of human hemoglobin in

Correct AnswerverifiedShow Answer

Restriction endonucleases were discovered by

Correct AnswerverifiedShow Answer

Recombinant DNA technology does not rely on which of the following enzymes?

Correct AnswerverifiedShow Answer

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
True

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
D

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified