Question 1

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.
Section 2.02

Accepted Answer

A) True 
 B)False

Question 2

A 30 ×	imes×  objective and a 20 ×	imes×  ocular produce a total magnification of

Accepted Answer

A)   230 ×	imes× .
B)   320 ×	imes× .
C)   50 ×	imes× .
D)   600 ×	imes× . E) A) and C)
F) B) and C)

Question 3

An electron microscope uses __________ lenses to focus beams of electrons onto a specimen.

Accepted Answer

The answer of An electron microscope uses __________ lenses to...

Question 4

Mordants increase the binding between a stain and specimen.

Accepted Answer

A) True 
 B)False

Question 5

Confocal microscopes exhibit improved contrast and resolution by

Accepted Answer

A)   illumination of a large area of the specimen.
B)   blocking out stray light with an aperture located above the objective lens.
C)   use of light at longer wavelengths.
D)   use of ultraviolet light to illuminate the specimen.E) B) and C)
F) A) and D)

Question 6

Atomic force microscopes use a scanning probe that maintains a fixed distance from the surface of the specimen. It is useful for specimens that

Accepted Answer

A)   do not conduct electricity well.
B)   have extremely uneven surfaces.
C)   both do not conduct electricity well and have extremely uneven surfaces are correct.
D)   neither do not conduct electricity well nor have extremely uneven surfaces is correct.E) A) and B)
F) None of the above

Question 7

Which of the following is considered to be a differential staining procedure?

Accepted Answer

A)   Gram stain
B)   Acid-fast stain
C)   both Gram stain and Acid-fast stain
D)   Leifson's flagella stainE) B) and C)
F) B) and D)

Question 8

Prior to staining, smears of microorganisms are heat-fixed in order to

Accepted Answer

A)   allow eventual visualization of internal structures.
B)   ensure removal of dust particles from the slide surface.
C)   attach it firmly to the slide.
D)   create small pores in cells that facilitates binding of stain to cell structures.Section 2.03E) A) and D)
F) A) and B)

Question 9

Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

Accepted Answer

A)   differential interference contrast
B)   dark field
C)   phase-contrast
D)   confocalE) All of the above
F) C) and D)

Question 10

After the decolorizer has been added, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Accepted Answer

A)   purple; purple
B)   purple; colorless
C)   purple; pink
D)   pink; pinkE) All of the above
F) C) and D)

Question 11

In the Gram-staining procedure, the decolorizer is

Accepted Answer

A)   iodine.
B)   safranin.
C)   crystal violet.
D)   ethanol or acetone.E) All of the above
F) B) and C)

Question 12

In the Gram-staining procedure, the primary stain is

Accepted Answer

A)   iodine.
B)   safranin.
C)   crystal violet.
D)   alcohol.E) B) and C)
F) A) and D)

Question 13

If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Accepted Answer

A)   purple; blue
B)   purple; colorless
C)   purple; pink
D)   pink; pinkE) A) and C)
F) None of the above

Question 14

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized.
Section 2.02

Accepted Answer

The answer of The useful magnification of a light microscope...

Question 15

In order to stain flagella so that they may be readily observed by light microscopy, it is usually necessary to increase their thickness.

Accepted Answer

A) True 
 B)False

Question 16

It is possible to build a light microscope capable of 10,000$\times$ magnification, but the image would not be sharp because resolution is independent of magnification.

Accepted Answer

A) True 
 B)False

Question 17

The procedure in which a single stain is used to visualize microorganisms is called __________ staining.

Accepted Answer

The answer of The procedure in which a single stain...

Question 18

In the Gram-staining procedure, the mordant is

Accepted Answer

A)   iodine.
B)   safranin.
C)   crystal violet.
D)   alcohol.E) A) and B)
F) B) and D)

Question 19

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

Accepted Answer

A)   phase-contrast
B)   dark-field
C)   scanning electron
D)   fluorescenceE) None of the above
F) B) and C)

Question 20

The designer of the first transmission electron microscope, _________________, was awarded the 1986 Nobel Prize in physics.

Accepted Answer

The answer of The designer of the first transmission electron...

Exam 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

The procedure in which a single stain is used to visualize microorganisms is called __________ staining.

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In the Gram-staining procedure, the decolorizer is

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In the Gram-staining procedure, the mordant is

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The designer of the first transmission electron microscope, _________________, was awarded the 1986 Nobel Prize in physics.

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An electron microscope uses __________ lenses to focus beams of electrons onto a specimen.

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Mordants increase the binding between a stain and specimen.

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Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

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Prior to staining, smears of microorganisms are heat-fixed in order to

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Which of the following is considered to be a differential staining procedure?

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It is possible to build a light microscope capable of 10,000 ×\times× magnification, but the image would not be sharp because resolution is independent of magnification.

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The useful magnification of a light microscope is limited by the ___________ of the light source being utilized. Section 2.02

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A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm. Section 2.02

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A 30 ×\times× objective and a 20 ×\times× ocular produce a total magnification of

Correct AnswerverifiedShow Answer

In order to stain flagella so that they may be readily observed by light microscopy, it is usually necessary to increase their thickness.

Correct AnswerverifiedShow Answer

Atomic force microscopes use a scanning probe that maintains a fixed distance from the surface of the specimen. It is useful for specimens that

Correct AnswerverifiedShow Answer

After the decolorizer has been added, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

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If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Correct AnswerverifiedShow Answer

Confocal microscopes exhibit improved contrast and resolution by

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In the Gram-staining procedure, the primary stain is

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A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

Correct AnswerverifiedShow Answer

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It is possible to build a light microscope capable of 10,000 $\times$ magnification, but the image would not be sharp because resolution is independent of magnification.

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A 30 $\times$ objective and a 20 $\times$ ocular produce a total magnification of

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After the decolorizer has been added, gram-positive organisms are stained and gram-negative organisms are stained .

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If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained and gram-negative organisms will be stained .

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