Question 1

What is the - 35 consensus sequence for the following sequences?

Accepted Answer

A)   ACAA
B)   TTGATA
C)   TATGAT
D)   TATTAT
E)   TAGTATF) A) and B)
G) D) and E)

Question 2

You want to design a drug that prevents transcription of eukaryotic mRNAs but does not affect transcription of other RNAs. What enzyme would you target?

Accepted Answer

A)   RNA polymerase III
B)   RNA polymerase I
C)   ribozyme
D)   methyl transferase
E)   RNA polymerase IIF) A) and C)
G) A) and B) E

Question 3

Describe the mechanism(s) used by bacteria and eukaryotes for transcription termination.

Accepted Answer

Bacterial transcription termination can ...

Question 4

One type of RNA editing involves inserting uracils into edited mRNA with the assistance of which type of specialized RNA?

Accepted Answer

A)   telomerase RNA
B)   guide RNA
C)   post- transcriptional editor RNA
D)   transposon RNA
E)   small nucleolar RNAF) A) and B)
G) A) and E)

Question 5

What proteins are bound to enhancers, forming a protein "bridge" that bends the DNA over the promoter to initiate transcription?

Accepted Answer

The answer of What proteins are bound to enhancers, forming...

Question 6

What are the mechanisms for transcription termination in bacteria?

Accepted Answer

intrinsic ...

Question 7

Which region(s)  of a gene are NOT found within the mRNA transcript?

Accepted Answer

A)   promoter and termination region
B)   promoter region
C)   stop codon
D)   termination region
E)   promoter and stop codonF) B) and E)
G) B) and D) A

Question 8

Which of the following is part of a DNA molecule?

Accepted Answer

A)   sigma
B)   activator
C)   RNA polymerase
D)   promoter
E)   transcription factorF) D) and E)
G) C) and D)

Question 9

Polyadenylation begins with the binding of _________near a six- nucleotide mRNA sequence, AAUAAA, downstream of the stop codon.

Accepted Answer

cleavage and polyade...

Question 10

RNA polymerase I transcribes which tandem- repeat genes in nucleoli?

Accepted Answer

A)   mRNA
B)   tRNA
C)   siRNA
D)   rRNA
E)   all types of RNAF) A) and E)
G) A) and B)

Question 11

What are two distinguishing features of RNA?

Accepted Answer

A)   RNA has a ribose sugar and uracil nitrogenous base.
B)   RNA contains phophodiester bonds as part of its sugar backbone.
C)   RNA forms a double helix of reverse complementary strands.
D)   RNA contains a methylated form of thymine.
E)   RNA contains a pyrophosphate group bound to the ribose.F) B) and E)
G) C) and D)

Question 12

A cell has a defect in polyadenylation of mRNA. The RNA transcripts encoding which type of protein would NOT be affected by this defect because they are not polyadenylated?

Accepted Answer

A)   SR proteins
B)   histone proteins
C)   DNA binding proteins
D)   transcription factors
E)   transmembrane proteinsF) A) and B)
G) A) and C)

Question 13

What is the purpose of alternative splicing in eukaryotic cells?

Accepted Answer

A)   Improve the efficiency of transcription and translation.
B)   Regulate the quantity of any single protein being produced in the cell.
C)   Increase the number of genes that do not have to contain introns.
D)   Produce multiple types of tRNAs that can bind to different codons.
E)   Produce multiple polypeptide sequences from a single primary transcript.F) All of the above
G) B) and D)

Question 14

Which of the following statements accurately describes tRNA?

Accepted Answer

A)   tRNAs are a variety of lengths and fold into a variety of shapes.
B)   Amino acids are bound to the 5' end of the tRNA.
C)   Wobble in the anticodon allows a single tRNA to bind to multiple codons.
D)   Post- transcriptional modifications of tRNAs are not necessary for their function.
E)   All organisms produce tRNAs corresponding to the 61 amino- acid coding codons.F) All of the above
G) A) and E)

Question 15

Processing of the 30S pre- RNA transcript in humans produces which three rRNAs after enzymatic cleavage?

Accepted Answer

The answer of Processing of the 30S pre- RNA transcript...

Question 16

Which three types of RNAs are found in both prokaryotes and eukaryotes?

Accepted Answer

mRNA, tRNA...

Question 17

Describe the three main differences between bacterial and eukaryotic mRNA transcripts.

Accepted Answer

• Eukaryotic mRNA transcripts are more s...

Question 18

In the following prokaryotic DNA sequence, label the highlighted regions.
 
 Using the prokaryotic DNA sequence above, describe the relationship between the highlighted regions and how mutations in each region may affect gene expression.

Accepted Answer

The - 10 (Pribnow) and - 35 consensus se...

Question 19

The rat a- tropomyosin (a- Tm)  gene produces nine different mature mRNA proteins from a single gene using which three "alternative" mechanisms?

Accepted Answer

A)   5' cap, branch points, polyadenylation
B)   5' cap, self- splicing, polyadenylation
C)   splicing, start codon, stop codon
D)   promoters, start codon, poly(
A)   signal sequences
E)   splicing, promoters, and polyadenylationF) A) and D)
G) B) and E) E

Question 20

RNA polymerase is called a_______, meaning it is an intact complex with full enzymatic capacity.

Accepted Answer

The answer of RNA polymerase is called a_______, meaning it...

Exam 8: Molecular Biology of Transcription and Rna Processing

What is the - 35 consensus sequence for the following sequences?

Correct AnswerverifiedShow Answer

You want to design a drug that prevents transcription of eukaryotic mRNAs but does not affect transcription of other RNAs. What enzyme would you target?

Correct AnswerverifiedE

Describe the mechanism(s) used by bacteria and eukaryotes for transcription termination.

Correct AnswerverifiedBacterial transcription termination can ...View AnswerShow Answer

One type of RNA editing involves inserting uracils into edited mRNA with the assistance of which type of specialized RNA?

Correct AnswerverifiedShow Answer

What proteins are bound to enhancers, forming a protein "bridge" that bends the DNA over the promoter to initiate transcription?

Correct AnswerverifiedShow Answer

What are the mechanisms for transcription termination in bacteria?

Correct Answerverifiedintrinsic ...View AnswerShow Answer

Which region(s) of a gene are NOT found within the mRNA transcript?

Correct AnswerverifiedA

Which of the following is part of a DNA molecule?

Correct AnswerverifiedShow Answer

Polyadenylation begins with the binding of _________near a six- nucleotide mRNA sequence, AAUAAA, downstream of the stop codon.

Correct Answerverifiedcleavage and polyade...View AnswerShow Answer

RNA polymerase I transcribes which tandem- repeat genes in nucleoli?

Correct AnswerverifiedShow Answer

What are two distinguishing features of RNA?

Correct AnswerverifiedShow Answer

A cell has a defect in polyadenylation of mRNA. The RNA transcripts encoding which type of protein would NOT be affected by this defect because they are not polyadenylated?

Correct AnswerverifiedShow Answer

What is the purpose of alternative splicing in eukaryotic cells?

Correct AnswerverifiedShow Answer

Which of the following statements accurately describes tRNA?

Correct AnswerverifiedShow Answer

Processing of the 30S pre- RNA transcript in humans produces which three rRNAs after enzymatic cleavage?

Correct AnswerverifiedShow Answer

Which three types of RNAs are found in both prokaryotes and eukaryotes?

Correct AnswerverifiedmRNA, tRNA...View AnswerShow Answer

Describe the three main differences between bacterial and eukaryotic mRNA transcripts.

Correct Answerverified• Eukaryotic mRNA transcripts are more s...View AnswerShow Answer

In the following prokaryotic DNA sequence, label the highlighted regions. Using the prokaryotic DNA sequence above, describe the relationship between the highlighted regions and how mutations in each region may affect gene expression.

Correct AnswerverifiedThe - 10 (Pribnow) and - 35 consensus se...View AnswerShow Answer

The rat a- tropomyosin (a- Tm) gene produces nine different mature mRNA proteins from a single gene using which three "alternative" mechanisms?

Correct AnswerverifiedE

RNA polymerase is called a_______, meaning it is an intact complex with full enzymatic capacity.

Correct AnswerverifiedShow Answer

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E

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Bacterial transcription termination can ...
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intrinsic ...
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A

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cleavage and polyade...
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mRNA, tRNA...
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• Eukaryotic mRNA transcripts are more s...
View Answer

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The - 10 (Pribnow) and - 35 consensus se...
View Answer

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E

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