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Processing of pre-tRNAs to produce tRNAs involves


A) cleavage of the pre-tRNA.
B) CCA addition to the 5ʹ end.
C) phosphodiesterase activity to cleave the base and deoxyribose.
D) the removal of one intron.

E) A) and C)
F) B) and C)

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A process called alternative splicing of mRNA transcripts can produce mRNAs with _______ from the same gene.


A) one different exon
B) more or fewer exons
C) completely different exons
D) All of the above

E) A) and B)
F) A) and C)

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The RNA components of spliceosomes are five types of _______.

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snRNAs (sm...

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Eukaryotic RNA polymerase I genes code for


A) mRNAs.
B) tRNAs.
C) small nuclear RNAs and small cytoplasmic RNAs.
D) ribosomal RNAs.

E) B) and C)
F) A) and D)

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The large multi-subunit complex that links the general transcription factors to the gene-specific transcription factors is called


A) the transcription complex.
B) Mediator.
C) the operator.
D) TBP.

E) None of the above
F) A) and B)

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Processing of tRNAs involves addition of a CCA 5′ terminus.

A) True
B) False

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mRNAs are degraded in the cytoplasm by


A) shortening of the poly-A tail and degradation from the 3′ end by nucleases.
B) removal of the 5′ cap and degradation from the 5′ end by nucleases.
C) Both a and b
D) Neither a nor b

E) A) and B)
F) None of the above

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All of the following are general transcription factors used in eukaryotic transcription except


A) TFIIE
B) TFIIH
C) TBP
D) BRE

E) A) and B)
F) A) and C)

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Eukaryotic RNA polymerase III synthesizes _______.

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Suppose that a gene has three exons and two introns in the following order: 5ʹ exon 1 - intron 1 - exon 2 - intron 2 - exon 3 3ʹ Which of the following could not result from alternative splicing in this gene?


A) 5ʹ exon 1 - exon 2 - exon 3 3ʹ
B) 5ʹ exon 2 - exon 3 3ʹ
C) 5ʹ exon 2 - exon 1 3ʹ
D) 5ʹ exon 1 - exon 3 3ʹ

E) C) and D)
F) B) and D)

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Which statement about transcriptional termination in prokaryotes is false?


A) Termination is signaled by transcription of a GC-rich interval.
B) Transcription terminates when the RNA polymerase dissociates from its DNA template.
C) The ribosome comes to a UAA, UAG, or UGA stop codon, and transcription ceases.
D) A segment of RNA forms a stable stem-loop structure by complementary base pairing.

E) A) and C)
F) C) and D)

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What important information was revealed by the discovery of catalytic RNAs?

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Biologists had long puzzled over the nee...

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Release of RNA polymerase II to initiate transcription appears to be the direct result of the


A) binding of TAFs to the polymerase.
B) unwinding of the DNA by helicases.
C) phosphorylation of RNA polymerase by a protein kinase.
D) removal of the nucleosome occupying the promoter site.

E) B) and C)
F) A) and C)

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Processing of RNA transcripts occurs


A) only in eukaryotic cells.
B) only with mRNA transcripts.
C) only with rRNA and mRNA transcripts.
D) with tRNA, rRNA, and mRNA transcripts.

E) A) and B)
F) None of the above

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Explain why the lariat structure that is formed as an intermediate in RNA splicing could not be formed in a DNA molecule.

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The first step of splicing is cleavage a...

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RNA splicing takes place in large complexes called _______.

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The σ\sigma subunit of E. coli RNA polymerase is necessary for transcriptional elongation.

A) True
B) False

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The processing of pre-rRNA requires proteins and RNAs that are localized to the nucleolus.

A) True
B) False

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Which of the following RNAs is RNA polymerase II not responsible for transcribing?


A) mRNA
B) miRNA
C) tRNA
D) lncRNA

E) All of the above
F) None of the above

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The DNA sequence to which an RNA polymerase binds to initiate transcription of a gene is called a(n)


A) enhancer.
B) promoter.
C) polymerase-binding element.
D) origin of transcription.

E) None of the above
F) B) and C)

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