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What disease does Clostridium perfringens cause?


A) Botulism
B) Tetanus
C) Food poisoning
D) Toxic shock syndrome

E) A) and B)
F) B) and D)

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Aerotolerant anaerobes include all the following EXCEPT:


A) Clostridium
B) Bacteroides
C) Actinomyces
D) Bifidobacterium

E) A) and B)
F) A) and C)

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A microbiology technologist is reading an anaerobic culture and observes a double zone of hemolysis on an anaerobically incubated sheep blood agar plate.The Gram stain of that organism was a boxcar-shaped,gram-positive bacillus.What organism is this?


A) Clostridium perfringens
B) C. difficile
C) C. ramosum
D) C. clostridiiforme

E) B) and D)
F) B) and C)

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What disk is used to presumptively identify Peptostreptococcus anaerobius?


A) Bile
B) Nitrate
C) Sodium polyanethol sulfonate
D) Indole

E) All of the above
F) None of the above

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All of the following techniques are used for making definitive identification of anaerobes EXCEPT:


A) PRAS and non-PRAS tubed biochemical test media
B) Gas-liquid chromatography of metabolic end products
C) Preexisting enzyme-based minisystems
D) Mass spectroscopy

E) C) and D)
F) A) and B)

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Which of the following is considered a better specimen for anaerobic culture than a swab?


A) Aspirate
B) Washing
C) Scraping
D) Lavage

E) A) and B)
F) B) and C)

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What substances do organisms that use oxygen have to protect themselves from superoxide anions?


A) Hyaluronidase and proteases
B) Exotoxins and superoxide dismutase
C) Enterotoxins and lipase
D) Superoxide dismutase and catalase

E) A) and B)
F) A) and C)

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How are suspected colonies of anaerobes processed?


A) Gram stain morphology and reaction is observed.
B) An aerotolerance test is set up.
C) A pure culture/subculture plate is inoculated and appropriate disks are added.
D) All of the above.

E) B) and C)
F) A) and C)

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What organism mostly commonly causes gas gangrene?


A) Clostridium difficile
B) C. tetani
C) C. perfringens
D) C. botulinum

E) None of the above
F) B) and D)

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How long are anaerobic cultures routinely held in the laboratory?


A) 1 to 2 days
B) 3 to 4 days
C) 5 to 7 days
D) 7 to 9 days

E) A) and C)
F) C) and D)

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An immunosuppressed patient notices sinus tracts that are draining pus.He also notices that there appear to be small,hard "nuggets" in the pus.What disease will his doctor most likely diagnose?


A) Gas gangrene
B) Pseudomembranous colitis
C) Actinomycosis
D) Myonecrosis

E) C) and D)
F) A) and C)

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Gram stains of specimens for anaerobic cultures should be examined for all the following reasons EXCEPT:


A) The Gram stain reveals various types and relative number of bacteria present.
B) Certain morphotypes may provide a presumptive identification and will guide media selection.
C) The Gram stain often reveals the presence of leukocytes.
D) The Clinical and Laboratory Standards Institute (CLSI) guidelines recommend Gram stains on all anaerobic specimens.

E) None of the above
F) B) and C)

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All of the following are approaches to treating anaerobic infections EXCEPT:


A) Surgical therapy
B) Hyperbaric oxygen
C) Antitoxins
D) Enzyme inactivators

E) B) and D)
F) A) and D)

Correct Answer

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All of the following are non-spore-forming,anaerobic,gram-positive bacilli EXCEPT:


A) Actinomyces
B) Bifidobacterium
C) Eubacterium
D) Clostridium

E) All of the above
F) A) and B)

Correct Answer

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