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Viral replication occurs


A) intracellularly.
B) extracellularly.
C) both intracellularly and extracellularly.
D) either intracellularly or extracellularly, depending on the virus involved.

E) B) and D)
F) B) and C)

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The use of ________ is the easiest and most effective way of studying many animal and plant viruses.


A) bacterial cultures
B) tissue or cell culture
C) live hosts
D) prophages

E) B) and D)
F) B) and C)

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Penetration requires that the entire virus is inserted within the host.

A) True
B) False

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Rolling circle replication of the lambda genome differs from replication of a bacterial chromosome in that


A) bidirectional replication forks are not formed.
B) only a single strand of the genome is copied.
C) no concatamers are formed.
D) only a single strand of the genome is copied and no concatamers are formed.

E) A) and D)
F) A) and C)

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Ocean and lake water typically contain 10⁷ virions/mL.Does this mean that swimming in or drinking this water will make you sick? Why or why not? How do these viruses impact human and environmental health?

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Although there are many virions per mL o...

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Which statement is TRUE?


A) All viruses contain their own nucleic acid polymerases.
B) Many viruses contain their own nucleic acid polymerases.
C) Viruses do not contain their own nucleic acid polymerases.
D) The origins of the nucleic acid polymerases used by viruses are eukaryotic.

E) B) and C)
F) A) and D)

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B

Viruses have both an intracellular and an extracellular form.

A) True
B) False

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In a natural population of diverse slow-growing prokaryotic cells,what type of viruses would you expect to be most common?


A) lytic bacteriophages
B) enveloped viruses
C) icosahedral viruses
D) temperate bacteriophages

E) A) and B)
F) None of the above

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Explain why some viruses contain enzymes within the virion and others do not.Use specific examples to support your answer.

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In the example of retroviruses,the rever...

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In viruses,genetic information flows from ________ to ________.


A) RNA / virion
B) nucleic acid / protein
C) capsid / virion
D) DNA / protein

E) All of the above
F) A) and D)

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RNA viruses encode host restriction systems designed to destroy host DNA.

A) True
B) False

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Viruses can confer additional properties on their host cells,which can in turn be inherited.

A) True
B) False

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You are attempting to mutate lambda to affect whether lysis or lysogeny occurs after lambda infection.Which mutation would INCREASE the chances of LYSOGENY over lysis?


A) deletion or inactivation of the cI gene
B) deletion or inactivation of the cro gene
C) overexpression of the cro gene
D) deletion of both the cro and cI genes

E) C) and D)
F) None of the above

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Tailed bacterial viruses can be used as genetic engineering tools.

A) True
B) False

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The virus repressor protein provides immunity to infection by the same type of virus.

A) True
B) False

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The latent phase in the viral growth curve and the lag phase of the bacterial growth curve are equivalent and represent the time it takes for the virus or bacterium to adapt to the culture conditions and begin growing.

A) True
B) False

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Lysogeny is unique to bacteriophages; similar relationships have not been found among the animal viruses.

A) True
B) False

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Describe the establishment and maintenance of the lysogenic state in the lambda phage.

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Lambda phage,once circularized by matchi...

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The growth of viruses in a culture is described as a one-step growth curve,because


A) virion numbers show no increase during intracellular replication and can only be counted after the virions burst from the host cell.
B) there is only one step in the viral life cycle which leads to only one replicative cycle in a culture.
C) assembly and release actually occur in one step.
D) the eclipse phase prevents the plating and enumeration of virions although new virions are produced at a steady rate during the eclipse phase.

E) A) and C)
F) B) and D)

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A

Relate the structure of bacteriophages and animal viruses to the structure of their respective host cells and the steps of the viral life cycle.

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The T4 phage has a very complex structure,containing a head,collar,tail,tail pins,an endplate,and tail fibers.The tail fibers bind to specific receptors on the surface of a bacterial cell (outside the peptidoglycan cell wall).When the tail fibers retract,the tail pins bind to the host so that the tail itself interacts with the cell wall.A viral enzyme then forms a small pore in the host cell wall.The tail's covering then contracts and inserts the virus into the host's cytoplasm.Animal viruses are usually much simpler in structure than bacteriophages because animal host cells lack a cell wall.Penetration is therefore somewhat easier and is usually accomplished by endocytosis or merging of the viral envelope with the host cell membrane.The complicated fibers and contractile tail are not needed to penetrate the animal cell.One last difference is that the entire virion often enters the animal cell and not just the viral genome.

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