Question 1

In their famous experiment, which of the following would Meselson and Stahl have observed after one cycle of replication in 14N medium if DNA replication was conservative?

Accepted Answer

A)  DNA molecules containing one strand of 15N-DNA and one strand of 14N-DNA
B)  DNA molecules containing two 15N-DNA strands only
C)  DNA molecules containing two 14N-DNA strands only
D)  An equal number of DNA molecules containing two 15N-DNA strands and DNA molecules containing two 14N-DNA strands.
E)  A mix of DNA molecules corresponding to A and B.F) A) and B)
G) A) and C)

Question 2

What is removed from dNTPs to provide the energy required for DNA polymerase to catalyze DNA strand elongation?

Accepted Answer

two phosph...

Question 3

Which enzyme, a combination of several proteins and a molecule of tRNA, acts as the template for the telomere repeat DNA sequence?

Accepted Answer

The answer of Which enzyme, a combination of several proteins...

Question 4

What types of bonds are formed between complementary DNA bases?

Accepted Answer

A)  glycosidic bonds
B)  phosphodiester bonds
C)  covalent bonds
D)  hydrogen bonds
E)  ionic bondsF) A) and B)
G) A) and C)

Question 5

Based on the following replication bubble, which of these statements is true?

Accepted Answer

A)  X and Y are leading strands, W and Z are lagging strands
B)  X and Z are leading strands, W and Y are lagging strands
C)  X and W are leading strands, Y and Z are lagging strands
D)  W and Z are leading strands, X and Y are lagging strands
E)  W and Y are leading strands, X and Z are lagging strandsF) B) and C)
G) B) and D)

Question 6

What structure in E. coli delivers primase and accessory proteins to the oriC site to synthesize the primer needed for DNA replication?

Accepted Answer

The answer of What structure in E. coli delivers primase...

Question 7

A portion of one strand of DNA has the sequence 5′ AATGGCTTA 3′. If this strand is used as a template for DNA replication, which of the following correctly depicts the sequence of the newly synthesized strand in the direction in which it will be synthesized?

Accepted Answer

A)  3′ TTACCGAAT 5′
B)  5′ TAAGCCATT 3′
C)  3′ AATGGCTTA 5′
D)  5′ AATGGCTTA 3′E) C) and D)
F) A) and D)

Question 8

What are the three steps of each PCR cycle?

Accepted Answer

denaturati...

Question 9

What type of experimental technique did Huberman and Riggs use to produce the first evidence of bidirectional replication in bacteria?

Accepted Answer

pulse-chas...

Question 10

Which functional groups have been altered in a ddNTP compared to a dNTP?

Accepted Answer

A)  The ddNTPs have a 2′ OH and a 3′ H, while dNTPs have a 2′ H and a 3′ OH.
B)  The ddNTPs have a 2′ H and a 3′ H, while dNTPs have a 2′ H and a 3′ OH.
C)  The ddNTPs have a 2′ OH and a 3′ OH, while dNTPs have a 2′ H and a 3′ H.
D)  The ddNTPs have a 2′ H and a 3′ H, while dNTPs have a 2′ OH and a 3′ OH.
E)  The ddNTPs have a 2′ H and a 3′ OH, while dNTPs have a 2′ H and a 3′ H.F) A) and B)
G) A) and E)

Question 11

What type of bond is formed between the hydroxyl group of one nucleotide and the phosphate group of an adjacent nucleotide, forming the sugar-phosphate backbone of DNA?

Accepted Answer

A)  glycosidic bond
B)  phosphodiester bond
C)  ester linkage
D)  hydrogen bond
E)  ionic bondF) C) and D)
G) None of the above

Question 12

What enzyme(s) is/are responsible for removal of RNA primers and joining of Okazaki fragments?

Accepted Answer

A)  DNA polymerase I
B)  DNA polymerase III
C)  DNA ligase
D)  DNA polymerase I and DNA ligase
E)  DNA polymerase III and DNA ligaseF) B) and E)
G) A) and D)

Question 13

How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

Accepted Answer

look for h...

Question 14

What are the regions where DNA-binding proteins can make direct contact with exposed nucleotides?

Accepted Answer

major and ...

Question 15

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension) ?

Accepted Answer

A)  95° → 72° → 55°
B)  45° → 72° → 95°
C)  95° → 55° → 72°
D)  95° → 72° → 95°
E)  75° → 95° → 45°F) B) and C)
G) All of the above

Question 16

In E. coli, which protein uses ATP to hydrolyze hydrogen bonds holding complementary strands together?

Accepted Answer

The answer of In E. coli, which protein uses ATP...

Question 17

The following represents a DNA strand in the process of replication. The bottom sequence is that of the DNA strand with polarity indicated and the top sequence represents the RNA primer. GGGGCCUUU
5′ AAATCCCCGGAAACTAAAC 3′
Which of the following will be the first DNA nucleotide added to the primer?

Accepted Answer

A)  A
B)  G
C)  C
D)  T
E)  UF) All of the above
G) A) and E)

Question 18

If complementary DNA strands were arranged in a parallel manner, what would you expect to see?

Accepted Answer

A)  Complementary nucleotides would be attracted to each other, forming ionic bonds that would make the helix stable but not uniform in width.
B)  Charges of complementary nucleotides would repel and no hydrogen bonds would form.
C)  Complementary nucleotides would line up properly; but fewer hydrogen bonds would form, so the strands could be more easily pulled apart.
D)  Purines would pair with both purines and pyrimidines due to the closer alignment of the parallel strands.
E)  There would be no discernable difference between DNA strands aligned in a parallel versus an antiparallel manner.F) B) and C)
G) B) and E)

Question 19

In 1928, Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

Accepted Answer

A)  living type RII
B)  heat-killed type SIII
C)  heat-killed type RII
D)  heat-killed type SIII and living type RII
E)  heat-killed type RII and living type RIIF) C) and D)
G) None of the above

Question 20

What are the three parts of a DNA nucleotide?

Accepted Answer

deoxyribos...

Exam 7: Dna Structure and Replication

In 1928, Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

Correct Answer
verified

Which functional groups have been altered in a ddNTP compared to a dNTP?

Correct Answer
verified

Based on the following replication bubble, which of these statements is true?

Correct Answer
verified

What are the three steps of each PCR cycle?

Correct Answer
verified
denaturati...
View Answer

In E. coli, which protein uses ATP to hydrolyze hydrogen bonds holding complementary strands together?

Correct Answer
verified

In their famous experiment, which of the following would Meselson and Stahl have observed after one cycle of replication in ¹⁴N medium if DNA replication was conservative?

Correct Answer
verified

If complementary DNA strands were arranged in a parallel manner, what would you expect to see?

Correct Answer
verified

A portion of one strand of DNA has the sequence 5′ AATGGCTTA 3′. If this strand is used as a template for DNA replication, which of the following correctly depicts the sequence of the newly synthesized strand in the direction in which it will be synthesized?

Correct Answer
verified

Which enzyme, a combination of several proteins and a molecule of tRNA, acts as the template for the telomere repeat DNA sequence?

Correct Answer
verified

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension) ?

Correct Answer
verified

How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

Correct Answer
verified
look for h...
View Answer

What are the three parts of a DNA nucleotide?

Correct Answer
verified
deoxyribos...
View Answer

What type of bond is formed between the hydroxyl group of one nucleotide and the phosphate group of an adjacent nucleotide, forming the sugar-phosphate backbone of DNA?

Correct Answer
verified

What are the regions where DNA-binding proteins can make direct contact with exposed nucleotides?

Correct Answer
verified
major and ...
View Answer

What type of experimental technique did Huberman and Riggs use to produce the first evidence of bidirectional replication in bacteria?

Correct Answer
verified
pulse-chas...
View Answer

Correct Answer
verified

What structure in E. coli delivers primase and accessory proteins to the oriC site to synthesize the primer needed for DNA replication?

Correct Answer
verified

What types of bonds are formed between complementary DNA bases?

Correct Answer
verified

What enzyme(s) is/are responsible for removal of RNA primers and joining of Okazaki fragments?

Correct Answer
verified

What is removed from dNTPs to provide the energy required for DNA polymerase to catalyze DNA strand elongation?

Correct Answer
verified
two phosph...
View Answer

Exam 7: Dna Structure and Replication

In 1928, Griffith found that injecting which strains of Pneumococcus caused the mouse to die?

Correct AnswerverifiedShow Answer

Which functional groups have been altered in a ddNTP compared to a dNTP?

Correct AnswerverifiedShow Answer

Based on the following replication bubble, which of these statements is true?

Correct AnswerverifiedShow Answer

What are the three steps of each PCR cycle?

Correct Answerverifieddenaturati...View AnswerShow Answer

In E. coli, which protein uses ATP to hydrolyze hydrogen bonds holding complementary strands together?

Correct AnswerverifiedShow Answer

In their famous experiment, which of the following would Meselson and Stahl have observed after one cycle of replication in 14N medium if DNA replication was conservative?

Correct AnswerverifiedShow Answer

If complementary DNA strands were arranged in a parallel manner, what would you expect to see?

Correct AnswerverifiedShow Answer

A portion of one strand of DNA has the sequence 5′ AATGGCTTA 3′. If this strand is used as a template for DNA replication, which of the following correctly depicts the sequence of the newly synthesized strand in the direction in which it will be synthesized?

Correct AnswerverifiedShow Answer

Which enzyme, a combination of several proteins and a molecule of tRNA, acts as the template for the telomere repeat DNA sequence?

Correct AnswerverifiedShow Answer

Which of the following temperature cycles would you expect to see in a standard polymerase chain reaction (from denaturation to annealing to extension) ?

Correct AnswerverifiedShow Answer

How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

Correct Answerverifiedlook for h...View AnswerShow Answer

What are the three parts of a DNA nucleotide?

Correct Answerverifieddeoxyribos...View AnswerShow Answer

What type of bond is formed between the hydroxyl group of one nucleotide and the phosphate group of an adjacent nucleotide, forming the sugar-phosphate backbone of DNA?

Correct AnswerverifiedShow Answer

What are the regions where DNA-binding proteins can make direct contact with exposed nucleotides?

Correct Answerverifiedmajor and ...View AnswerShow Answer

What type of experimental technique did Huberman and Riggs use to produce the first evidence of bidirectional replication in bacteria?

Correct Answerverifiedpulse-chas...View AnswerShow Answer

Correct AnswerverifiedShow Answer

What structure in E. coli delivers primase and accessory proteins to the oriC site to synthesize the primer needed for DNA replication?

Correct AnswerverifiedShow Answer

What types of bonds are formed between complementary DNA bases?

Correct AnswerverifiedShow Answer

What enzyme(s) is/are responsible for removal of RNA primers and joining of Okazaki fragments?

Correct AnswerverifiedShow Answer

What is removed from dNTPs to provide the energy required for DNA polymerase to catalyze DNA strand elongation?

Correct Answerverifiedtwo phosph...View AnswerShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
denaturati...
View Answer

Correct Answer
verified

In their famous experiment, which of the following would Meselson and Stahl have observed after one cycle of replication in ¹⁴N medium if DNA replication was conservative?

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
look for h...
View Answer

Correct Answer
verified
deoxyribos...
View Answer

Correct Answer
verified

Correct Answer
verified
major and ...
View Answer

Correct Answer
verified
pulse-chas...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
two phosph...
View Answer